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multiplex identifier  (Illumina Inc)


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    Illumina Inc multiplex identifier
    Multiplex Identifier, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+identifier/Nextera+XT+Index+Kit/10__1080_slash_21683565__2024__2419408-69-16-21
    Average 99 stars, based on 3944 article reviews
    multiplex identifier - by Bioz Stars, 2026-09
    99/100 stars

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    Sequencing:

    Article Title: Does an agroecological approach impact the nematode biological and functional diversity in intensive protected production systems?
    Article Snippet: Food production systems in protected conditions are probably the most intensive agricultural systems causing biodiversity loss.. Experimental sites in different European areas were established to investigate whether an agroecological approach to organic greenhouse production allows preserving or increasing biodiversity.. Soil nematode communities were characterized by DNA-metabarcoding, and some biodiversity indices were calculated.

    Article Title: Increased N2O emissions by the soil nematode community cannot be fully explained by enhanced mineral N availability
    Article Snippet: Soil nematodes are the most abundant soil fauna, with a potential great impact on soil N mineralization via interaction with soil microorganisms.. As a consequence, nematodes likely also influence soil N2O production and emission but the very few studies on this matter were carried out in simplified setups with single nematode species and in (highly) disturbed soil conditions.. Here we measured soil N2O emissions in a 74-day incubation experiment in the presence or absence of the entire soil nematode community with minimal disturbance of the soil microbial community and soil nutrients.

    Article Title: New Insights Into Nematode DNA-metabarcoding as Revealed by the Characterization of Artificial and Spiked Nematode Communities
    Article Snippet: .. Each index primer was composed of a sequence specific for Illumina sequencing, a unique 8 bp multiplex identifier (N7xx or S5xx, Illumina Nextera XT Index Kit v2, Illumina Inc., San Diego, CA, USA) and the Illumina adapter overhang sequence. ..

    Multiplex Assay:

    Article Title: Does an agroecological approach impact the nematode biological and functional diversity in intensive protected production systems?
    Article Snippet: Food production systems in protected conditions are probably the most intensive agricultural systems causing biodiversity loss.. Experimental sites in different European areas were established to investigate whether an agroecological approach to organic greenhouse production allows preserving or increasing biodiversity.. Soil nematode communities were characterized by DNA-metabarcoding, and some biodiversity indices were calculated.

    Article Title: Increased N2O emissions by the soil nematode community cannot be fully explained by enhanced mineral N availability
    Article Snippet: Soil nematodes are the most abundant soil fauna, with a potential great impact on soil N mineralization via interaction with soil microorganisms.. As a consequence, nematodes likely also influence soil N2O production and emission but the very few studies on this matter were carried out in simplified setups with single nematode species and in (highly) disturbed soil conditions.. Here we measured soil N2O emissions in a 74-day incubation experiment in the presence or absence of the entire soil nematode community with minimal disturbance of the soil microbial community and soil nutrients.

    Article Title: New Insights Into Nematode DNA-metabarcoding as Revealed by the Characterization of Artificial and Spiked Nematode Communities
    Article Snippet: .. Each index primer was composed of a sequence specific for Illumina sequencing, a unique 8 bp multiplex identifier (N7xx or S5xx, Illumina Nextera XT Index Kit v2, Illumina Inc., San Diego, CA, USA) and the Illumina adapter overhang sequence. ..



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    Image Search Results


    Samples used as positive controls to validate the deep amplicon  sequencing  approach for the accurate detection of species composition.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Amplification, Sequencing, Cell Culture

    Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Sequencing

    Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Sequencing